Cell Culture Basics Handbook

The handbook and videos are intended as an introduction to cell culture basics.
The first four chapters of the handbook focus on cell culture, covering topics
such as getting familiar with the requirements of a laboratory dedicated to
cell culture experiments, laboratory safety, aseptic technique, and microbial
contamination of cell cultures, as well as providing basic methods for passaging,
freezing, and thawing cultured cells. The subsequent two chapters of the
handbook focus on various transfection technologies and provide general
guidelines for the selection of the appropriate transfection method, the
transfection of cells with plasmid DNA, oligonucleotides, and RNA, as well
as culture preparation for in vitro and in vivo transfection and selection of the
transfected cells. 

The information and guidelines presented in the handbook and the instructional
videos focus on cell lines (finite or continuous) and omit experiments and
techniques concerning primary cultures and stem cells, such as isolating and
disaggregating tissues, reprogramming cells into pluripotent stem cells, or
differentiating stem cells into various lineages.